Khawaja will stay with the squad, the team said, keeping alive his hopes of extending his Test career past his 39th birthday next month.
Spin-bowling all-rounder Will Jacks will replace paceman Mark Wood in an otherwise unchanged England side for the second Ashes Test against Australia in Brisbane later this week, the team said on Tuesday.
Wood experienced soreness in his left knee after the series opener in Perth, which Australia won by eight wickets inside two days to take a 1-0 lead in the five-match series.
Even if he failed to take a wicket, the 35-year-old's pace was a big plus for England in the first innings in Perth but his enduring health was always in question given he missed the entire home summer because of a problem with the same knee.
The inclusion of Jacks for his first Test since he won his second cap in Pakistan in 2022 marks a move away from the four-pronged pace attack England put out in Perth, despite the Gabba also being renowned for its fast, bouncy surface.
Although Jacks took 6-161 on his test debut in Pakistan three years ago, he is better known for his prowess with the bat and will be expected to stiffen the bottom end of England's batting order.
Zak Crawley has retained his spot at the top of the England batting order despite picking up a pair in Perth, a decision consistent with the view of coach Brendon McCullum and captain Ben Stokes that the tourists should stick to their guns.
The second Test, a day-nighter, begins in Brisbane on Thursday.
England: Zak Crawley, Ben Duckett, Ollie Pope, Joe Root, Harry Brook, Ben Stokes (captain), Jamie Smith, Will Jacks, Gus Atkinson, Brydon Carse, Jofra Archer
Australia opener Khawaja ruled out of second Ashes Test
Australia opener Usman Khawaja has been ruled out of the second Ashes Test against in England in Brisbane later this week by a back problem, the team said on Tuesday.
Khawaja was unable to open either innings in the series opener in Perth because of back spasms and showed some discomfort on Monday in the nets at Brisbane's Gabba ground, where the second Test starts on Thursday.
The 38-year-old will not be replaced in the squad, raising the likelihood that Travis Head will stay at the top of the order after his brilliant innings of 123 as a makeshift opener in Perth drove Australia to an eight-wicket victory.
Marnus Labuschagne and Steve Smith have both been used as openers in the nearly two years since David Warner retired, but the former said on Monday that neither would be reprising the role.
Khawaja will stay with the squad, the team said, keeping alive his hopes of extending his Test career past his 39th birthday next month.