Dorisse, Lucinda, and KitKat are part of a nature recovery programme at the site.
The meadows, a Site of Special Scientific Interest (SSSI), are owned by Vale of White Horse District Council and managed by Earth Trust with help from Friends of Tuckmill volunteers.
The reserve is home to around 150 plant species and includes habitats for various birds and mammals, such as otters and water voles.
The cows were introduced at the end of summer to graze the site and limit the fast-growing grasses.
This ensures more diverse and delicate plant species can thrive, including four species of conservation concern: field scabious, ragged robin, hoary plantain, and marsh valerian.
Tim Read, Earth Trust countryside manager for Tuckmill Meadows, said: "The variety of terrain at Tuckmill - the limestone valley, the fenland, and the stream - contributes to the diverse species found here, but it does make it difficult to mow with machinery.
"In the past, the meadows were used for grazing and this kept the grass in check.
"When grazing was stopped, volunteers cut vegetation by hand but had to focus on key areas as it's slow going."
The Dexter cows, being smaller and lighter than other cattle breeds, are well-suited to the terrain.
They have GPS tags on their collars, allowing site managers to track their movements and prevent them from entering unsuitable areas.
The tags create an "invisible" fence, emitting a musical tune if the cows move towards a restricted area.
The information from the collars supplements regular visits to check on the animals' welfare, with Earth Trust now seeking volunteer stock watchers.
It is anticipated that the cattle will remain on site year-round, but the footpath alongside the golf course boundary will be kept cattle-free.
Councillor Hayleigh Gascoigne, Vale of White Horse District Council's cabinet member for environmental services, climate action, and nature recovery, said: "We have had a long association with the Earth Trust managing our countryside sites using a variety of measures to get the best possible outcome for our local nature.
"We are grateful to them and all the hard work put in by local volunteers to protect and nurture this wonderful green space."
District ward councillor Katherine Foxhall said: "This is such an important site for nature and for the residents of Shrivenham and Watchfield, many of whom visit regularly to enjoy the variety of plants and birds.
"We hope the cattle will provide yet another reason for people to enjoy the natural beauty of Tuckmill Meadows."